2 edition of Regulation of expression of the chitinase gene (CTSI) in Saccharomyces cerevisiaeeby Lorraine King. found in the catalog.
Regulation of expression of the chitinase gene (CTSI) in Saccharomyces cerevisiaeeby Lorraine King.
Lorraine M. King
|Contributions||University College Dublin. Department of Biochemistry.|
|The Physical Object|
|Pagination||(10), 113, (15)p., several p.ages of plates ;|
|Number of Pages||113|
relative level of expression of the target gene was calculated according to the 2- (ΔΔ Ct) method of Livak and Schmittgen (). Cloning the full-length promoter of the peanut chitinase gene Three nested specific primers (SP1, SP2, and SP3) were designed using the 5'-end sequence of peanut chitinase cDNA (GenBank accession No. HQ).Cited by: 3. as a probe, the tissue specificity and hormonal regulation of expression of the chitinase gene during development were studied (Kramer et al., ). In order to extend our understanding of the structure of chitinase genes in insects and to help determine how they are regulated,Cited by:
T1 - Cloning and expression of a chitinase gene from Thermoactinomyces vulgaris KFB-C AU - Yoon, Ho Geun. AU - Kim, Hee Yun. AU - Lim, Young Hee. AU - Cho, Hong Yon. PY - /12/1. Y1 - /12/1. N2 - We have found that Thermoactinomyces vulgaris KFB-C produces a chitinase. The optimum temperature and pH of the enzyme activity were To better understand the molecular regulation of defense responses in members of the genus Pinus, we tested the expression of various chitinase homologs in response to pathogen-associated 4, a putative extracellular class II chitinase, was secreted into liquid medium by pine cells and was also secreted by transgenic tobacco cells that ectopically expressed pschi4.
Insect chitinases are hydrolytic enzymes that are required for the degradation of glycosidic bonds of chitin. In this study, we identified and characterized a full-length cDNA of the chitinase gene (BdCht2) in the oriental fruit fly, Bactrocera dorsalis. The cDNA contains an open reading frame (ORF) of bp that encodes amino acid residues and and bp non-coding regions at the 5. Plants synthesize a number of antimicrobial proteins in response to pathogen invasion and environmental stresses. These proteins include two classes of chitinases that have either basic or acidic isoelectric points and that are capable of degrading fungal cell wall chitin. We have cloned and determined the nucleotide sequence of the genes encoding the acidic and basic chitinases from.
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The high levels of chitinase activity in habitual smokers result from upregulation of CHIT1 gene expression, especially in macrophages. The higher chitotriosidase (ChT) activity in milk of mothers of premature infants than those of full-term infants suggests the presence of Regulation of expression of the chitinase gene book macrophages as the main source of ChT.
Chitinases are also present in plants (barley seed chitinase: EC ); some of these are pathogenesis related (PR) proteins that are induced as part of systemic acquired resistance. Expression is mediated by the NPR1 gene and the salicylic acid pathway, both BRENDA: BRENDA entry.
Regulation of chitinase 33 (chit33) gene expression in Trichoderma harzianum Article (PDF Available) in Current Genetics 38(6) February with Reads How we measure 'reads'. Chitinase expression in the insect gut normally occurs only during moulting, where the chitin of the peritrophic membrane is presumably degraded.
The data normalized to endogenous reference gene were presented as the fold-change in gene expression during different stages of infection and relative to the levels of expression observed at 1 day after infection.
Ifu-chit2expression levels peaked at 2 days after infection (Figure 6). Neither chitinase gene was expressed in uninoculated insects. Hevea brasiliensis, the largest commercial source of natural rubber, is known to be invaded by multiple fungal pathogens, affecting the global rubber production resulting in severe economic losses.
Usually, chitinases form the very first line of defense against fungal pathogens in plants. However, the chitinase gene family structure remains elusive in the rubber tree genome.
A new basic chitinase gene, designated RC24, was isolated from a rice genomic library. The predicted RC24 protein contains amino acid residues and exhibits 68% to 95% amino acid identity with known class I rice chitinases.
RC24 protein expressed in Escherichia coli exhibited chitinase activity and strongly inhibited bacterial growth. Two transcription start sites of the RC24 gene were Cited by: Chitinase 1 (CHIT1) The human chitinase gene (CHIT1) has been assigned to chromosome 1q31–q32, and its gene product is a circulating enzyme also known as plasma chitotriosidase or methylumbelliferyl tetra-N-acetylchitotetraoside hydrolase.
This enzyme is excreted by macrophages and is believed to play a role in degrading chitin, which. Here, we provide evidence, within the E. grandis genome assembly, for 67 chitinase gene models within two families: glycosyl hydrolase 18 and We note that, although the E.
grandis genome has an expanded chitinase gene family, only a single putative Class IA chitinase is present, unlike the genomes of P. trichocarpa and H. by: 7. In other words, the chitinase genes that originated from the same A. thaliana gene showed similar expression patterns. After inoculation with P.
brassicae, 14 of the 33 chitinase genes were induced in the roots, hypocotyls, or leaves and used for further analysis (Fig. 5).Cited by: Chitinase gene expression has been shown to be transcriptionally regulated by a number of inducers, including ethylene, elicitors, and pathogen attack.
To investigate the mechanism(s) responsible for induction of chitinase gene expression in response to various stimuli, we have developed a transient gene expression system in bean (Phaseolus vulgaris) protoplasts that is responsive to Cited by: To identify the chitinase gene involved in drought and low temperature responses in A.
nanus, we performed genome-wide identification, classification, sequence alignment, and spatio-temporal gene expression analysis of the chitinases in A.
nanus under osmotic and low temperature by: 2. Abstract. We describe the cloning and characterization of a single copy gene from Trichoderma atroviride P1 encoding a novel 30 kDa chitinase, Ech Ech30 is a family 18 chitinase showing low sequence similarity to other Trichoderma chitinases.
Real-time quantitative RT-PCR studies revealed that expression of the ech30 gene was induced by the presence of Botrytis cinerea in plate Cited by: Transcription from thechiC promoter, directing expression of the chitinase gene,chiC, inStreptomyces coelicolor, was analyzed usingxylE reporter gene and high-resolution S1-nuclease mapping.
The transcription from thechiC promoter was induced by chitin, and this induction was dramatically reduced in theS. coelicolor chiR-disrupted strain.
This indicated a dependence ofchiC Cited by: Expression of at least two genes from bean encoding the defense-related protein chitinase has been shown previously to be transcriptionally regulated by the phytohormone ethylene. We have determined the complete nucleotide sequence of one of these genes, the CH5B gene, which resides on a kilobase fragment of bean genomic DNA.
The structural gene consists of a single open reading Cited by: Regulation of expression may be estrogen-dependent. Gene expression and protein secretion occur during late follicular development through early cleavage-stage embryonic development. The protein is secreted from non-ciliated oviductal epithelial cells and associates with ovulated oocytes, blastomeres, and spermatozoon acrosomal regions.
Aliases: OVGP1, CHIT5, EGP, MUC9, OGP. Insect chitinases are hydrolytic enzymes that are required for the degradation of glycosidic bonds of chitin. In this study, we identified and characterized a full-length cDNA of the chitinase gene (BdCht2) in the oriental fruit fly, Bactrocera dorsalis.
The cDNA contains an open reading frame (ORF) of bp that encodes amino acid residues and and bp non-coding regions at the 5 Cited by: Cloning and expression analysis of the chitinase gene Ifu-chit2from Isaria fumosorosea Huimin Meng1,#, Zhangxun Wang1,#, Xiangyun Meng1, Ling Xie2 and Bo Huang1 1Anhui Provincial Key Laboratory of Microbial Pest Control, Anhui Agricultural University, Hefei, China.
2School of Life Sciences, Anqing Teachers College, Anqing, China Abstract Entomopathogenic fungi can produce a series of. The data normalized to endogenous reference gene were presented as the fold-change in gene expression during different stages of infection and relative to the levels of expression observed at 1 day after infection.
Ifu-chit2 expression levels peaked at 2 days after infection. Neither chitinase gene was expressed in uninoculated by: 2. The data strongly demonstrated that bacterial dsRNA could silence the endogenous expression of chitinase genes in gut tissue of M.
separata. Detection of chitinase gene specific siRNAs. RNAi effects are not only signified by a decline in the target mRNA level, but also by the accumulation of siRNAs with sequence-specific by:.
VI. Bacterial Chitinase Genes VII. Expression of a Bacterial Chitinase Gene in Plant Cells VIII. Activity of a Bacterial Chitinase Gene in Plants IX. Conclusions References 11 Structure and Regulation of Organ- and Tissue-Specific Genes: Structural and Cytological Features of Incompatibility Gene Expression in Flowering Plants I.
Introduction Edition: 1. A lack of empirical evidence for the microbial regulation of ecosystem processes, including carbon (C) degradation, hinders our ability to develop a framework to Cited by: Co-expression of a rice basic chitinase gene (RCH10) and a modified maize ribosome-inactivating protein (MOD1) in transgenic rice significantly enhanced resistance to sheath blight Cited by: